Set in the early 24th century against the backdrop of the city of Neo Venezia on the planet Mars. No longer the barren red planet, Mars has been flooded, inhabited and is something of a tourist hub for those looking for rest, relaxation and a gondola ride – the primary mode of travel in Neo Venezia. The Undines are professional gondoliers, tour guides for the people passing through. Akari Mizunashi is an Undine in training and this is a piece of her story.

New CE 2012. the mysterious organization Vagras aims to threaten the city by stealing the Enetron which is a new power source supporting people’s life. It's up to the Go-Busters to protect the city alongside their partners, the Buddyroids.

In the world of Daikuuriku, everyone is born female, and chooses which sex they wish to become at age 17. In this world, the peaceful theocracy of Simulacrum is guarded by magical flying machines called "Simoun," which can only be piloted by young girls who haven't chosen a sex yet. When the industrialized nation of Argentum decides that it needs to invade Simulacrum to acquire the secret of the Simoun, war breaks out, drawing the Simoun Sibyllae into a lopsided battle.

This V-Cinema release takes place between Missions 44 & 45. While preparing to celebrate New Year's, the Go-Busters are all killed by a monster named Azazel. Finding himself (and J) before God in Heaven, Nick uses his status as the one millionth person to die in 2012 to grant his wish that Messiah never existed. He awakens to find a changed world, where the Busters are now known as the Dobutsu Sentai Go-Busters.