John Howland travels to the frozen North to build a branch of the Hudson Bay Railroad. There he meets and falls in love with Meleese Thoreau who warns him that her three bloodthirsty brothers, Max, Pierre and François, have sworn vengeance against a man named John Howland, the son of a man who killed their mother, and that torture and death await him along the route to his station.

Wah-Ta-Wah, or Hist, the lady-love of Chingachgook, a Delaware chief, has been captured by the warlike Hurons. Chingachgook asks the aid of Deerslayer, a white man brought up among the Indians, in rescuing her, and. the two men arrange to meet at Lake Otsego, then called Glimmerglass. Deerslayer sets out for the meeting place, accompanied by Hurry Harry March, a trapper, who acts as his guide.

A husband, mistakenly believing his wife has cheated on him and that he is now the father of their newborn son, throws both her and her child out of the house. Frantic to the point of madness, she abandons her baby, and when she gains her sanity she flees to Alaska to start a new life. However, her husband finds out and follows her there.