In post-9/11 New York City, an eclectic group of citizens find their lives entangled, personally, romantically, and sexually, at Shortbus, an underground Brooklyn salon infamous for its blend of art, music, politics, and carnality.

Beautiful vampire Djuna tries to resist the advances of the handsome, human screenwriter Paolo, but eventually gives in to their passion. When her seductive and highly volatile sister Mimi unexpectedly comes to visit, she threatens Djuna's new relationship, and the whole vampire community becomes endangered.

After finally breaking up with his drug-addicted lover, Trevor begins a new romance that unexpectedly complicates his other relationships.

A look at the unusual process used in the making of the film Shortbus (2006) featuring interviews, behind the scenes footage and clips from the feature film. Director John Cameron Mitchell starts with the concept of using real sex in a film with a positive message. The cast of unknowns is selected from homemade audition tapes and then a callback audition workshop. More acting workshops are used to develop the characters and script. The project overcomes a number of obstacles and the rest of the film's development is followed up until its premiere at the Cannes Film Festival.