Two transfer students, Atsuko Maeda and Onizuka Daruma came at the all-girls school Majisuka Gakuen. In this school violence and fighting are common among the Yankee students. Noisy and trouble maker Daruma made a fuss as soon as she entered the school, win in her first fight but then was beaten easily by another gang shortly after. In contrary to Daruma's bold-but-powerless attitude, Maeda who is truthfully a strong girl despite of her silent nature found Daruma is beaten by Team Hormon before her. The word "Majisuka?" (means are you serious?) instantly awaken her true nature and made her won the battle against Daruma's attackers easily, thus made a big fuss at the school and the news even reached the strongest fighting group, Rappapa. Afterwards, Daruma acknowledged Maeda as her boss and as a result the friendship between the two girls began to grow along with their fights with fellow students at their school.

Libby, a female basketball player, is caught in confusion when she is faced with two boys who are quite different in age from her and who both love Libby.